The wood grain and panel joins are clearly visible in the x-ray image in the centre, which also shows the soft blending of the paint in the face. The two filled holes in the sitter's forehead, which can be seen in both the x-ray and the infrared reflectogram on the right, are probably nail holes from an old hanging method. There is minimal underdrawing; the lines that mark out the features are painted above other paint layers. Marks beneath the pendant pearl may indicate that it was originally planned to hang lower down.

© National Portrait Gallery, London